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Image Search Results
Journal: Communications Biology
Article Title: One-step Cre- loxP organism creation by TAx9
doi: 10.1038/s42003-025-07759-9
Figure Lengend Snippet: a Genomic PCR for the floxed site in F1 heterozygous KI mice (intact, 2.5 kbp; recombined, 1.5 kbp). These mice were injected with three doses (3D) or six doses (6D) of tamoxifen or the solvent corn oil alone (TAM−) two weeks before limb sample collection ( n = 3 each). Lane numbers indicate the ID of the individual. WT: genomic DNA of a wild-type C57BL/6J mouse. P: plasmid DNA (control). M: size marker. Pink arrowheads: 1.5 kbp (recombined). b − e Representative images showing tamoxifen-dependent induction of mCherry expression in SMF cells ( n = 3 for each). F1 heterozygous KI mice were injected with either six doses of tamoxifen (TAM+) or the solvent corn oil alone (TAM−). The limb samples were cross-sectioned in the middle of the zeugopod (20 µm thick). mCherry fluorescence was detected in the TAM+ mice ( b , c ) but not in the TAM− mice ( d , e ). Note that EGFP fluorescence in the TAM+ mice was weaker than in the TAM− mice, suggesting recombination of the floxed site. The optical sections (1.3–2.0 µm thick) of the boxed areas of the merged images in ( b ) and ( d ), acquired by laser confocal microscopy, are shown in ( c ) and ( e ), respectively. Scale bars: b and d 1 mm for upper panels, 200 µm for lower panel; c and e 100 µm.
Article Snippet: Genomic DNA was obtained from the tail tips of larval newts (St. 59) and mice (1 month old) with a
Techniques: Injection, Solvent, Plasmid Preparation, Control, Marker, Expressing, Fluorescence, Confocal Microscopy